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Mosquitocidal Effect of Glycosmis pentaphylla Leaf Extracts against Three Mosquito Species (Diptera: Culicidae)

Identifieur interne : 000118 ( Main/Exploration ); précédent : 000117; suivant : 000119

Mosquitocidal Effect of Glycosmis pentaphylla Leaf Extracts against Three Mosquito Species (Diptera: Culicidae)

Auteurs : Govindaraju Ramkumar [Inde] ; Sengodan Karthi [Inde] ; Ranganathan Muthusamy [Inde] ; Ponnusamy Suganya [Inde] ; Devarajan Natarajan [Inde] ; Eliningaya J. Kweka [Tanzanie] ; Muthugounder S. Shivakumar [Inde]

Source :

RBID : PMC:4938602

Abstract

Background

The resistance status of malaria vectors to different classes of insecticides used for public health has raised concern for vector control programmes. Alternative compounds to supplement the existing tools are important to be searched to overcome the existing resistance and persistence of pesticides in vectors and the environment respectively. The mosquitocidal effects of Glycosmis pentaphylla using different solvents of acetone, methanol, chloroform and ethyl acetate extracts against three medically important mosquito vectors was conducted.

Methods

Glycosmis pentaphylla plant leaves were collected from Kolli Hills, India. The WHO test procedures for larval and adult bioassays were used to evaluate extracts against mosquito vectors, and the chemical composition of extracts identified using GC-MS analysis.

Results

The larvicidal and adulticidal activity of G. pentaphylla plant extracts clearly impacted the three species of major mosquitoes vectors. Acetone extracts had the highest larvicidal effect against An. stephensi, Cx. quinquefasciatus and Ae. aegypti with the LC50 and LC90 values of 0.0004, 138.54; 0.2669, 73.7413 and 0.0585, 303.746 mg/ml, respectively. The LC50 and LC90 adulticide values of G. pentaphylla leaf extracts in acetone, methanol, chloroform and ethyl acetate, solvents were as follows for Cx. quinquefasciatus, An. stephensi and Ae. Aegypti: 2.957, 5.458, 2.708, and 4.777, 3.449, 6.676 mg/ml respectively. The chemical composition of G. pentaphylla leaf extract has been found in 20 active compounds.

Conclusions

The plant leaf extracts of G. pentaphylla bioactive molecules which are effective and can be developed as an eco-friendly approach for larvicides and adulticidal mosquitoes vector control. Detailed identification and characterization of mosquitocidal effect of individual bioactive molecules ingredient may result into biodegradable effective tools for the control of mosquito vectors.


Url:
DOI: 10.1371/journal.pone.0158088
PubMed: 27391146
PubMed Central: 4938602


Affiliations:


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Le document en format XML

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<wicri:regionArea>Department of Medical Parasitology and Entomology, Catholic University of Health and Allied Sciences, P.O. Box 1464, Mwanza</wicri:regionArea>
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<name sortKey="Shivakumar, Muthugounder S" sort="Shivakumar, Muthugounder S" uniqKey="Shivakumar M" first="Muthugounder S." last="Shivakumar">Muthugounder S. Shivakumar</name>
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<title xml:lang="en" level="a" type="main">Mosquitocidal Effect of
<italic>Glycosmis pentaphylla</italic>
Leaf Extracts against Three Mosquito Species (Diptera: Culicidae)</title>
<author>
<name sortKey="Ramkumar, Govindaraju" sort="Ramkumar, Govindaraju" uniqKey="Ramkumar G" first="Govindaraju" last="Ramkumar">Govindaraju Ramkumar</name>
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<name sortKey="Suganya, Ponnusamy" sort="Suganya, Ponnusamy" uniqKey="Suganya P" first="Ponnusamy" last="Suganya">Ponnusamy Suganya</name>
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<name sortKey="Natarajan, Devarajan" sort="Natarajan, Devarajan" uniqKey="Natarajan D" first="Devarajan" last="Natarajan">Devarajan Natarajan</name>
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<nlm:aff id="aff003">
<addr-line>Division of Livestock and Human Diseases Vector Control, Mosquito Section Tropical Pesticides Research Institute, P.O. Box 3024, Arusha, Tanzania</addr-line>
</nlm:aff>
<country xml:lang="fr">Tanzanie</country>
<wicri:regionArea>Division of Livestock and Human Diseases Vector Control, Mosquito Section Tropical Pesticides Research Institute, P.O. Box 3024, Arusha</wicri:regionArea>
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</affiliation>
<affiliation wicri:level="1">
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<addr-line>Department of Medical Parasitology and Entomology, Catholic University of Health and Allied Sciences, P.O. Box 1464, Mwanza, Tanzania</addr-line>
</nlm:aff>
<country xml:lang="fr">Tanzanie</country>
<wicri:regionArea>Department of Medical Parasitology and Entomology, Catholic University of Health and Allied Sciences, P.O. Box 1464, Mwanza</wicri:regionArea>
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<name sortKey="Shivakumar, Muthugounder S" sort="Shivakumar, Muthugounder S" uniqKey="Shivakumar M" first="Muthugounder S." last="Shivakumar">Muthugounder S. Shivakumar</name>
<affiliation wicri:level="1">
<nlm:aff id="aff001">
<addr-line>Molecular Entomology Lab, Department of Biotechnology, Periyar University, Salem- 636 011, Tamil Nadu, India</addr-line>
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<country xml:lang="fr">Inde</country>
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<div type="abstract" xml:lang="en">
<sec id="sec001">
<title>Background</title>
<p>The resistance status of malaria vectors to different classes of insecticides used for public health has raised concern for vector control programmes. Alternative compounds to supplement the existing tools are important to be searched to overcome the existing resistance and persistence of pesticides in vectors and the environment respectively. The mosquitocidal effects of
<italic>Glycosmis pentaphylla</italic>
using different solvents of acetone, methanol, chloroform and ethyl acetate extracts against three medically important mosquito vectors was conducted.</p>
</sec>
<sec id="sec002">
<title>Methods</title>
<p>
<italic>Glycosmis pentaphylla</italic>
plant leaves were collected from Kolli Hills, India. The WHO test procedures for larval and adult bioassays were used to evaluate extracts against mosquito vectors, and the chemical composition of extracts identified using GC-MS analysis.</p>
</sec>
<sec id="sec003">
<title>Results</title>
<p>The larvicidal and adulticidal activity of
<italic>G</italic>
.
<italic>pentaphylla</italic>
plant extracts clearly impacted the three species of major mosquitoes vectors. Acetone extracts had the highest larvicidal effect against
<italic>An</italic>
.
<italic>stephensi</italic>
,
<italic>Cx</italic>
.
<italic>quinquefasciatus</italic>
and
<italic>Ae</italic>
.
<italic>aegypti</italic>
with the LC
<sub>50</sub>
and LC
<sub>90</sub>
values of 0.0004, 138.54; 0.2669, 73.7413 and 0.0585, 303.746 mg/ml, respectively. The LC
<sub>50</sub>
and LC
<sub>90</sub>
adulticide values of
<italic>G</italic>
.
<italic>pentaphylla</italic>
leaf extracts in acetone, methanol, chloroform and ethyl acetate, solvents were as follows for
<italic>Cx</italic>
.
<italic>quinquefasciatus</italic>
,
<italic>An</italic>
.
<italic>stephensi</italic>
and
<italic>Ae</italic>
.
<italic>Aegypti</italic>
: 2.957, 5.458, 2.708, and 4.777, 3.449, 6.676 mg/ml respectively. The chemical composition of
<italic>G</italic>
.
<italic>pentaphylla</italic>
leaf extract has been found in 20 active compounds.</p>
</sec>
<sec id="sec004">
<title>Conclusions</title>
<p>The plant leaf extracts of
<italic>G</italic>
.
<italic>pentaphylla</italic>
bioactive molecules which are effective and can be developed as an eco-friendly approach for larvicides and adulticidal mosquitoes vector control. Detailed identification and characterization of mosquitocidal effect of individual bioactive molecules ingredient may result into biodegradable effective tools for the control of mosquito vectors.</p>
</sec>
</div>
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<name sortKey="Natarajan, Devarajan" sort="Natarajan, Devarajan" uniqKey="Natarajan D" first="Devarajan" last="Natarajan">Devarajan Natarajan</name>
<name sortKey="Shivakumar, Muthugounder S" sort="Shivakumar, Muthugounder S" uniqKey="Shivakumar M" first="Muthugounder S." last="Shivakumar">Muthugounder S. Shivakumar</name>
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